Difference between revisions of "Part:BBa K1321336"

Revision as of 16:59, 21 October 2014

AraC-pBAD-AcsAB

This composite part is the result of combining parts BBa_K1321333 and BBa_K1321334 by BioBrick cloning. It contains the Arabinose-inducible promoter pBAD and also codes for the transcriptional inhibitor/activator AraC. Furthermore, pBAD is followed by the AcsAB open reading frame, which in turn codes for the cellulose synthase enzyme, key player for cellulose production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3049
Illegal BglII site found at 3763
Illegal BglII site found at 5686
Illegal BamHI site found at 1144
Illegal BamHI site found at 2059
Illegal BamHI site found at 5053
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
Illegal AgeI site found at 3035
Illegal AgeI site found at 3112
Illegal AgeI site found at 3668
Illegal AgeI site found at 4469
Illegal AgeI site found at 4561
Illegal AgeI site found at 4667
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1321336 short</partinfo>
-This part is made up of the Arabinose-inducible promoter pBAD and the coding sequence of its transcriptional inhibitor/activator AraC,  followed by the coding sequence of the A and B elements of the acs, cellulose-producing operon.
+This composite part is the result of combining parts BBa_K1321333 and BBa_K1321334 by BioBrick cloning. It contains the Arabinose-inducible promoter pBAD and also codes for the transcriptional inhibitor/activator AraC. Furthermore, pBAD is followed by the AcsAB open reading frame, which in turn codes for the cellulose synthase enzyme, key player for cellulose production.
 <!-- Add more about the biology of this part here