Difference between revisions of "Part:BBa K1321090:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1321090 short</partinfo>
 
<partinfo>BBa_K1321090 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Codon optimised for E.coli. TBC
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Codon optimised for E.coli.  
 +
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.
  
  
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===Source===
 
===Source===
  
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CBD2 is EnLinker-CBH2-EnLinker-CBH1 from T.reesei. TBC
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dCBD is EnLinker-CBH2-EnLinker-CBH1 from T.reesei (BBa_K1321340). Please refer to BBa_K1321005 for detailed information on synthetic phytochelatin EC(20).
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Both dCBD and the phytochelatin were designed and ordered from GeneArt® Gene (Invitrogen™ Life Technologies).
  
 
===References===
 
===References===

Revision as of 15:38, 21 October 2014

Phytochelatin (PC) EC20 + Linker-dCBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.


Source

dCBD is EnLinker-CBH2-EnLinker-CBH1 from T.reesei (BBa_K1321340). Please refer to BBa_K1321005 for detailed information on synthetic phytochelatin EC(20). Both dCBD and the phytochelatin were designed and ordered from GeneArt® Gene (Invitrogen™ Life Technologies).

References