Difference between revisions of "Part:BBa K1371015"

 
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<partinfo>BBa_K1371015 short</partinfo>
 
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== Substrate supply ==
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There are three enzymes needed in our chassis. Propionyl-CoA carboxylase(pcc), an enzyme needed in the biosynthesis of polyketides, is an enzyme complex containing two components playing the core role in our chassis, of which accA is the α biotinylated subunit functioning as the acetyl-CoA or propionyl-CoA carboxylase while pccB，the β subunit，acts as the carboxyl transferase【1】.
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== BirA ==
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BirA is a biotin ligase that improves the activity of accA, acts as an enhancer like sfp, the phosphopantetheinyl transferase facilitating posttranslational modification of the DEBS proteins in E.coli BL21(DE3) [2]. With the pcc and sfp genes transformed into E.coli BL21(DE3), it will express DEBS genes at the comparable level to S. erythraea or S.coelicolor [2].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:07, 21 October 2014

pT7+birA+accA+pccB+2TM-pT7+sfp+2TM

Substrate supply

There are three enzymes needed in our chassis. Propionyl-CoA carboxylase(pcc), an enzyme needed in the biosynthesis of polyketides, is an enzyme complex containing two components playing the core role in our chassis, of which accA is the α biotinylated subunit functioning as the acetyl-CoA or propionyl-CoA carboxylase while pccB，the β subunit，acts as the carboxyl transferase【1】.


BirA

BirA is a biotin ligase that improves the activity of accA, acts as an enhancer like sfp, the phosphopantetheinyl transferase facilitating posttranslational modification of the DEBS proteins in E.coli BL21(DE3) [2]. With the pcc and sfp genes transformed into E.coli BL21(DE3), it will express DEBS genes at the comparable level to S. erythraea or S.coelicolor [2].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2946
    Illegal BglII site found at 3362
    Illegal BamHI site found at 1489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1880
    Illegal NgoMIV site found at 1895
    Illegal NgoMIV site found at 3610
    Illegal NgoMIV site found at 3687
    Illegal AgeI site found at 2252
    Illegal AgeI site found at 2384
    Illegal AgeI site found at 2579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1712
    Illegal BsaI.rc site found at 2426
    Illegal BsaI.rc site found at 3826
    Illegal SapI.rc site found at 2039