Difference between revisions of "Part:BBa K1456003:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1456003=== | ===Applications of BBa_K1456003=== | ||
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| + | == SOD ASSAY == | ||
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| + | *Superoxide Dismutase 1 '''changes the oxygen radicals into 02 and H2O2'''. Using this feature of SOD we placed the oxygen radicals into the same solution as tetrazolium dye which uses the radicals as substrates and enabled them to compete. Hence this allowed us to measure the functional activity of SOD produced by our transfected cells using the spectrophotometer at 560 nm. | ||
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| + | <center style="width:400px;height:400px">https://static.igem.org/mediawiki/2014/b/b4/ATOMS-SOD2.jpg</center> | ||
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| + | [[Image:ATOMS-SOD6.jpg|400px|]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:53, 21 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1456003
SOD ASSAY
- Superoxide Dismutase 1 changes the oxygen radicals into 02 and H2O2. Using this feature of SOD we placed the oxygen radicals into the same solution as tetrazolium dye which uses the radicals as substrates and enabled them to compete. Hence this allowed us to measure the functional activity of SOD produced by our transfected cells using the spectrophotometer at 560 nm.
User Reviews
UNIQc493aed2240fefee-partinfo-00000000-QINU UNIQc493aed2240fefee-partinfo-00000001-QINU