Difference between revisions of "Part:BBa K1321334:Experience"

Revision as of 10:16, 21 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1321334

This part was cloned into part BBa_K1321333 by Biobrick cloning. The destination vector, containing the AraC-pBAD regulatory elements, was linearized using SpeI and PstI and AcsAB (previously digested with XbaI and PstI, was ligated at a 1:1 using the T4 ligase. The ligation mix was transformed into chemically competent DH10B Escherichia coli and plated on LB Agar+Chloramphenicol plates, grown overnight at 37 degrees celsius. 5 ml LB falcons supplied with 50 ug/ml Chloramphenicol were inoculated with a selection of freshly grown colonies for further restriction analysis (refer to Results for additional information)

User Reviews

UNIQcfb58cb155868f8f-partinfo-00000000-QINU UNIQcfb58cb155868f8f-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K1321334===
+This part was cloned into part  BBa_K1321333 by Biobrick cloning. The destination vector, containing the AraC-pBAD regulatory elements, was linearized using SpeI and PstI and AcsAB (previously digested with XbaI and PstI, was ligated at a 1:1 using the T4 ligase. The ligation mix was transformed into chemically competent DH10B Escherichia coli and plated on LB Agar+Chloramphenicol plates, grown overnight at 37 degrees celsius. 5 ml LB falcons supplied with 50 ug/ml Chloramphenicol were inoculated with a selection of freshly grown colonies for further restriction analysis (refer to Results for additional information)
 ===User Reviews===