Difference between revisions of "Part:BBa K1323017"
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<partinfo>BBa_K1323017 short</partinfo>
 
<partinfo>BBa_K1323017 short</partinfo>
  
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This part was created using the pSB1C3 backbone. The ''Staphylococcus'' oriV from the pSK41 plasmid [https://parts.igem.org/Part:BBa_K1323018 BBa_K1323018] and an erythromycin resistance gene [https://parts.igem.org/Part:BBa_K1323011 BBa_K1323011] was inserted between the VR and rep (pMB1) regions in pSB1C3 using BamHI and BglII restriction sites. The part contains an RFP cassette [https://parts.igem.org/Part:BBa_J04450 BBa_J04450] between the prefix and suffix.
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This part is an improvement of the [https://parts.igem.org/Part:pSB1C3:Experience pSB1C3 backbone]. This improvement makes the backbone much more versatile by turning into a functional <i> E. coli - Staphylococcus</i> shuttle vector. The ''Staphylococcus'' oriV from the pSK41 plasmid [https://parts.igem.org/Part:BBa_K1323018 BBa_K1323018] and an erythromycin resistance gene [https://parts.igem.org/Part:BBa_K1323011 BBa_K1323011] was inserted between the VR and rep (pMB1) regions in pSB1C3 using BamHI and BglII restriction sites. The part contains an RFP cassette [https://parts.igem.org/Part:BBa_J04450 BBa_J04450] between the prefix and suffix.
  
  

Revision as of 08:20, 21 October 2014

E. coli - Staphylococcus Shuttle Vector

This part is an improvement of the pSB1C3 backbone. This improvement makes the backbone much more versatile by turning into a functional E. coli - Staphylococcus shuttle vector. The Staphylococcus oriV from the pSK41 plasmid BBa_K1323018 and an erythromycin resistance gene BBa_K1323011 was inserted between the VR and rep (pMB1) regions in pSB1C3 using BamHI and BglII restriction sites. The part contains an RFP cassette BBa_J04450 between the prefix and suffix.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 4855
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4855
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4861
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4855
    Illegal BglII site found at 2978
    Illegal BamHI site found at 178
    Illegal BamHI site found at 186
    Illegal XhoI site found at 3839
    Illegal XhoI site found at 4731
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4855
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4855
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4870
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5657
    Illegal AgeI site found at 5769
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.