Difference between revisions of "Part:BBa K1321327:Design"
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<partinfo>BBa_K1321327 short</partinfo> | <partinfo>BBa_K1321327 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | BBa_K1321302 was created by restricting BBa_K1033917 (Anderson promoter+RFP insert) and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing. | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Revision as of 21:47, 20 October 2014
pTet02
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4629
Illegal SpeI site found at 890
Illegal PstI site found at 1773 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4629
Illegal SpeI site found at 890
Illegal PstI site found at 1773
Illegal NotI site found at 1766
Illegal NotI site found at 4635 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4629
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4629
Illegal SpeI site found at 890
Illegal PstI site found at 1773 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4629
Illegal XbaI site found at 4644
Illegal SpeI site found at 890
Illegal PstI site found at 1773
Illegal NgoMIV site found at 2948
Illegal AgeI site found at 1469
Illegal AgeI site found at 1581 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
BBa_K1321302 was created by restricting BBa_K1033917 (Anderson promoter+RFP insert) and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.