Difference between revisions of "Part:BBa K1321324:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
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BBa_K1321302 was created by restricting BBa_K1033903 (Anderson promoter+RFP insert) and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.
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BBa_K1321302 was created by restricting BBa_K1033903 and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.
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===Source===
 
===Source===

Revision as of 21:46, 20 October 2014

pLux01


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4785
    Illegal SpeI site found at 1046
    Illegal PstI site found at 1929
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4785
    Illegal SpeI site found at 1046
    Illegal PstI site found at 1929
    Illegal NotI site found at 1922
    Illegal NotI site found at 4791
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4785
    Illegal SpeI site found at 1046
    Illegal PstI site found at 1929
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4785
    Illegal XbaI site found at 4800
    Illegal SpeI site found at 1046
    Illegal PstI site found at 1929
    Illegal NgoMIV site found at 3104
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 1737
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


Design Notes

BBa_K1321302 was created by restricting BBa_K1033903 and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.

Source

References