Difference between revisions of "Part:BBa C0161:Experience"
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= Induction by the Supernatant of a LuxI Producing Culture = | = Induction by the Supernatant of a LuxI Producing Culture = | ||
| − | + | The production of an inducer for the [AHL|3OC6-HSL] and [Part:BBa_C0062|LuxR] dependent promoter [Part:BBa_R0062|R0062] by LuxI could be shown using the supernatant of a culture containing ''E. coli'' cells constitutively expressing LuxI to induce the [AHL|3OC6-HSL] sensor construct in another culture. | |
== Background information == | == Background information == | ||
Revision as of 21:38, 20 October 2014
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ETH Zurich 2014 |
Induction by the Supernatant of a LuxI Producing CultureThe production of an inducer for the [AHL|3OC6-HSL] and [Part:BBa_C0062|LuxR] dependent promoter [Part:BBa_R0062|R0062] by LuxI could be shown using the supernatant of a culture containing E. coli cells constitutively expressing LuxI to induce the [AHL|3OC6-HSL] sensor construct in another culture. Background informationExperimental SetupResults Figure 1: Dose-response curve LuxI producer supernatant The supernatant culture of E. coli cells in LB medium constitutively expressing LuxI was used in different dilutions with fresh LB medium as growth medium for E. coli cell containing a construct expressing sfGFP under a Plux promoter and constitutively expressing the regulator LuxR. Fluorescence/OD600 of sfGFP was measured 6 h after inoculation.
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Antiquity |
This review comes from the old result system and indicates that this part did not work in some test. |
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