Difference between revisions of "Part:BBa C0161:Experience"

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== Results ==
 
== Results ==
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[[File:ETH_Zurich_2014_LuxI_supernatant.png|500px|thumb|center|'''Figure 1: Dose-response curve LuxI producer supernatant'''  The supernatant culture of ''E. coli'' cells in LB medium constitutively expressing [[Part:BBa_C0161|LuxI]] was used in different dilutions with fresh LB medium as growth medium for ''E. coli'' cell containing a construct expressing sfGFP under a [[Part:BBa_R0062|Plux]] promoter and constitutively expressing the regulator [[Part:BBa_C0062|LuxR]]. Fluorescence of sfGFP was measured ]]
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[[File:ETH_Zurich_2014_LuxI_supernatant.png|500px|thumb|center|'''Figure 1: Dose-response curve LuxI producer supernatant'''  The supernatant culture of ''E. coli'' cells in LB medium constitutively expressing [[Part:BBa_C0161|LuxI]] was used in different dilutions with fresh LB medium as growth medium for ''E. coli'' cell containing a construct expressing sfGFP under a [[Part:BBa_R0062|Plux]] promoter and constitutively expressing the regulator [[Part:BBa_C0062|LuxR]]. Fluorescence/OD<sub>600</sub> of sfGFP was measured 6 h after inoculation.]]
  
  

Revision as of 21:33, 20 October 2014

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Applications of BBa_C0161

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ETH Zurich 2014

Induction by the Supernatant of a LuxI Producing Culture

Background information

Experimental Setup

Results

Figure 1: Dose-response curve LuxI producer supernatant The supernatant culture of E. coli cells in LB medium constitutively expressing LuxI was used in different dilutions with fresh LB medium as growth medium for E. coli cell containing a construct expressing sfGFP under a Plux promoter and constitutively expressing the regulator LuxR. Fluorescence/OD600 of sfGFP was measured 6 h after inoculation.



Antiquity

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