Difference between revisions of "Part:BBa K1321366:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was made from the composite parts that we submitted, and fused by Biobrick and Freiburg cloning. SfGFP was fused as a reporter for our CBD binding assay. | + | This part was made from the composite parts that we submitted, and fused by Biobrick and Freiburg cloning. SfGFP was fused as a reporter for our CBD binding assay, and may have uses for future teams as an easily quantifiable reporter. |
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===Source=== | ===Source=== | ||
Revision as of 20:47, 20 October 2014
CBDcex fused to sfGFP with T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 53
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 401
Design Notes
This part was made from the composite parts that we submitted, and fused by Biobrick and Freiburg cloning. SfGFP was fused as a reporter for our CBD binding assay, and may have uses for future teams as an easily quantifiable reporter.
Source
CBDcex comes from Cellulomonas fimi, and sfGFP is a modified super-folding mutant of GFP.