Difference between revisions of "Part:BBa K1456002:Experience"
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===Applications of BBa_K1456002=== | ===Applications of BBa_K1456002=== | ||
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| + | ===ATOMS TURKİYE 2014=== | ||
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| + | https://static.igem.org/mediawiki/2014/0/0e/ATOMS_gpx_results_16.jpg | ||
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| + | The amounts of protein acquired were as the charts above and the these data were taken into consideration in the preparation of the assays. | ||
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| + | https://static.igem.org/mediawiki/2014/d/df/ATOMS_gpx_results_17.jpg | ||
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| + | A '''functional assay''' of GPx was done through NADPH to measure GPx activity. The same amount of genes were transferred to all samples but varying amount of Na2SeO3 was prepared in the samples, with 2 samples containing different Na2SeO3 concentrations and one control group. The GPx gene was not transfected to the control group. From these 3 groups, the assay of the control group gave the lowest yield as expected; the sample of 500 nM Na2SeO3 produced the mid-result. The sample of 1000 nM Na2SeO3 yielded the highest activity results in the assay. These results prove that the GPx production is directly proportional to the amount of added Na2Se2O3 and that the GPx enzyme was produced functionally. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:31, 20 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1456002
ATOMS TURKİYE 2014
The amounts of protein acquired were as the charts above and the these data were taken into consideration in the preparation of the assays.
A functional assay of GPx was done through NADPH to measure GPx activity. The same amount of genes were transferred to all samples but varying amount of Na2SeO3 was prepared in the samples, with 2 samples containing different Na2SeO3 concentrations and one control group. The GPx gene was not transfected to the control group. From these 3 groups, the assay of the control group gave the lowest yield as expected; the sample of 500 nM Na2SeO3 produced the mid-result. The sample of 1000 nM Na2SeO3 yielded the highest activity results in the assay. These results prove that the GPx production is directly proportional to the amount of added Na2Se2O3 and that the GPx enzyme was produced functionally.
User Reviews
UNIQ2ce200f4c1ee5319-partinfo-00000000-QINU UNIQ2ce200f4c1ee5319-partinfo-00000001-QINU