Difference between revisions of "Part:BBa K1321327"

Revision as of 17:02, 20 October 2014

pTet02

This is a purple chromoprotein gfasPurple (BBa_K1033917) in pSEVA331-Bb plasmid backbone (part BBa_K1321300). This construct is a member of the G.xylinus genetic engineering toolbox.

G.xylinus toolbox was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium Gluconacetobacter xylinus (parts Bba_K1321305 and BBa_K1321306) by providing a collection of widely used parts in pSEVA331-Bb backbone. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in G.xylinus and E.coli (a shuttle vector) and was selected as the registry's standard plasmid backbone pSB1C3 can not be used for G.xylinus engineering.

NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in Gluconacetobacter species, the G.xylinus genetic engineering toolbox is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which can't be quality controlled and thus maintained by the registry. However, in order to make the G.xylinus toolbox available for the synthetic biology community, Imperial iGEM 2014 team has made the toolbox freely available upon request, with quality control provided (see Experience). For requests, please contact Michael Florea at mf1512@imperial.ac.uk.

Sequence and Features