Difference between revisions of "Part:BBa K1403015:Experience"
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The TMM enzyme is not specific to TMA as a substrate. It is also known to oxidize indole to indoxyl, which dimerizes into the well known blue pigment indigo (Fig 3B). Indole is a natural product of tryptophan metabolism in E. coli.
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The TMM enzyme is not specific to TMA as a substrate. It is also known to oxidize indole to indoxyl, which dimerizes into the well known blue pigment indigo. Indole is a natural product of tryptophan metabolism in E. coli.
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We took advangage of this indole production activity to characterize the TMM enzyme. E. coli that were cultured in LB supplemented with tryptophan (2 g/L) produced a deep blue pigment (Fig. 3C) with absorbance properties matching indigo (Fig. 4). Without TMM expression or without tryptophan, indigo production was minimal or absent.
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We took advangage of this indole production activity to characterize the TMM enzyme. E. coli that were cultured in LB supplemented with tryptophan (2 g/L) produced a deep blue pigment with absorbance properties matching indigo (Fig. 1). Without TMM expression or without tryptophan, indigo production was minimal or absent.
 
[[File:Spectrum_indigo.png|center|650px]]
 
[[File:Spectrum_indigo.png|center|650px]]

Revision as of 15:24, 20 October 2014

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UNIQ6333cd46157af48a-partinfo-00000000-QINU UNIQ6333cd46157af48a-partinfo-00000001-QINU The TMM enzyme is not specific to TMA as a substrate. It is also known to oxidize indole to indoxyl, which dimerizes into the well known blue pigment indigo. Indole is a natural product of tryptophan metabolism in E. coli. We took advangage of this indole production activity to characterize the TMM enzyme. E. coli that were cultured in LB supplemented with tryptophan (2 g/L) produced a deep blue pigment with absorbance properties matching indigo (Fig. 1). Without TMM expression or without tryptophan, indigo production was minimal or absent.

Spectrum indigo.png