Difference between revisions of "Part:BBa K1415203"

Revision as of 14:38, 20 October 2014

Pcons+B0034+PBAN(Agrotis ipsilon)+B0034+BFP+J61048

We use this part as reporter gene, it can check whether our PBAN works and quantify its expression.

Fig.1-1 Biobrick of Pcons + RBS + PBAN(AI) + BFP + Term.

Fig.1-2 Fig.1-2 The bluelight test of our E.coli

Fig.1-3 Modeling result of Pcons + RBS + PBAN(AI) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AI) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 909
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 [[File:Pcons(J23101) + RBS(B0034) + PBAN(AI) + RBS(B0034) + BFP(K592100) + Ter(J61048) .png|thumb|left|600px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(AI) + BFP + Term.]]
-[[File:BLAI.png|thumb|left|1200px|'''Fig.1-1''' Fig.1-2 The bluelight test of our E.coli]]
+[[File:BLAI.png|thumb|left|1200px|'''Fig.1-2''' Fig.1-2 The bluelight test of our E.coli]]
-[[File:MDAI.png|thumb|left|1200px|'''Fig.1-1''' Modeling result of Pcons + RBS + PBAN(AI) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AI) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.]]
+[[File:MDAI.png|thumb|left|1200px|'''Fig.1-3''' Modeling result of Pcons + RBS + PBAN(AI) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AI) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===