Difference between revisions of "Part:BBa K1321138"

Latest revision as of 12:47, 20 October 2014

Metallothionein SmtA with T7 promoter

SmtA is a metallotionein (metal binding peptide) from the cyanobacteria Synechococcus sp. and was first submitted by the Tokyo-NoKoGen 2011 iGEM team as BBa_K519010 (please refer to this page for detailed information on characteristion). Our version of the part has been made compatible with RFC25 fusion format at codon optimised for E. coli and is driven by the T7 promoter.

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 53
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1321138 short</partinfo>
-GATC
+SmtA is a metallotionein (metal binding peptide) from the cyanobacteria ''Synechococcus'' sp. and was first submitted by the Tokyo-NoKoGen 2011 iGEM team as BBa_K519010 (please refer to this page for detailed information on characteristion). Our version of the part has been made compatible with RFC25 fusion format at codon optimised for ''E. coli'' and is driven by the T7 promoter.
+Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
 <!-- Add more about the biology of this part here