Difference between revisions of "Part:BBa K1321123"

Latest revision as of 12:31, 20 October 2014

Metallothionein SmtA + linker-CBDcipA-linker

Metallothionein (metal binding peptide) SmtA fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous C and N-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 319
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 319
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 319
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 319
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 319
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1321123 short</partinfo>
-GATC
+Metallothionein (metal binding peptide) SmtA fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous C and N-terminal linker sequence.
+At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
+This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-SmtA-part-table.PNG]]
+Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
 <!-- Add more about the biology of this part here