Difference between revisions of "Part:BBa K1493703"

Revision as of 10:35, 20 October 2014

Part of CI induced toggle switch

Part of the BananaGuard CI inducible toggle switch. Combined with the BioBrick BBa_K1493702 this part forms a toggle switch containing a gfp reporter gene. This BioBrick is tested as the whole toggle switch. The part is assembled with BBa_K1493702 in a pSB3K3 backbone.

Figure 1. The relative fluorescence unit of each toggle switch state. Fluorescence is measured in duplo of cell cultures carrying the pSB3K3 plasmid with the toggle switch construct (BBa_K1493702, BBa_K1493703) grown in M9 medium containing 500 ng/ml aTc (green), 2 mM IPTG (red) and with no inducer added to the medium (blue).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1493703 short</partinfo>
-Part of the Banana Guard toggle switch. For the orther part of the toggle switch that combines with this part see BBa_K1493702.
+Part of the BananaGuard CI inducible toggle switch. Combined with the BioBrick BBa_K1493702 this part forms a toggle switch containing a gfp reporter gene.
+This BioBrick is tested as the whole toggle switch. The part is assembled with BBa_K1493702 in a pSB3K3 backbone.
+https://static.igem.org/mediawiki/2014/5/5a/Characterization_Toggle_Switch.png
+''Figure 1. The relative fluorescence unit of each toggle switch state. Fluorescence is measured in duplo of cell cultures carrying the pSB3K3 plasmid with the toggle switch construct (BBa_K1493702, BBa_K1493703) grown in M9 medium containing 500 ng/ml aTc (green), 2 mM IPTG (red) and with no inducer added to the medium (blue).''
 <!-- Add more about the biology of this part here