Difference between revisions of "Part:BBa K1321334"

Revision as of 10:31, 20 October 2014

pSB-AcsAB

This part contains the coding sequence, previously codon-optimised for expression in Escherichia coli, of the Cellulose Synthase enzyme. In Gluconacetobacter xylinus, the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such subunits. It is made up of two main domains, a catalytic domain (AcsA) and a regulatory domain (AcsB), which are transcribed and translated in the form of a single, functional unit with both regulatory and catalytic activities. The regulatory domain binds the second messenger c-di-GMP and activates the process, whilst the catalytic domain catalyses the polymerisation of cellulose from UDP-glucose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1831
Illegal BglII site found at 2545
Illegal BglII site found at 4468
Illegal BamHI site found at 841
Illegal BamHI site found at 3835
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1817
Illegal AgeI site found at 1894
Illegal AgeI site found at 2450
Illegal AgeI site found at 3251
Illegal AgeI site found at 3343
Illegal AgeI site found at 3449
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1321334 short</partinfo>
-This part contains the coding sequence, previously codon-optimised for expression in <em>Escherichia coli</em>, of the  Cellulose Synthase enzyme. In <em>Gluconacetobacter xylinus</em>,  the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such subunits. It is made up of two main domains, a catalytic domain (AcsA) and a regulatory domain (AcsB). AcsA and AcsB are transcribed and translated in the form of a single polypeptide
+This part contains the coding sequence, previously codon-optimised for expression in <em>Escherichia coli</em>, of the  Cellulose Synthase enzyme. In <em>Gluconacetobacter xylinus</em>,  the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such subunits. It is made up of two main domains, a catalytic domain (AcsA) and a regulatory domain (AcsB), which are transcribed and translated in the form of a single, functional unit with both regulatory and catalytic activities. The regulatory domain binds the second messenger c-di-GMP and activates the process, whilst the catalytic domain catalyses the polymerisation of cellulose from UDP-glucose.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===