Difference between revisions of "Part:BBa K1321334"
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<partinfo>BBa_K1321334 short</partinfo>
 
<partinfo>BBa_K1321334 short</partinfo>
  
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This part is made up of the coding sequence of the  Cellulose Synthase enzyme, which has been codon-optimised for expression in <em>Escherichia coli</em>. In <em>Gluconacetobacter xylinus</em>, this is contained within the Acs cellulose synthesis operon, a functional unit containing the essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such fundamental enzymes in Cellulose Production. It is made up of two main domains, a regulatory domain and a catalytic domain.
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This part contains the coding sequence, previously codon-optimised for expression in <em>Escherichia coli</em>, of the  Cellulose Synthase enzyme. In <em>Gluconacetobacter xylinus</em>, the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such subunits. It is made up of two main domains, a catalytic domain (AcsA) and a regulatory domain (AcsB). AcsA and AcsB are transcribed and translated in the form of a single polypeptide
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 10:26, 20 October 2014

pSB-AcsAB

This part contains the coding sequence, previously codon-optimised for expression in Escherichia coli, of the Cellulose Synthase enzyme. In Gluconacetobacter xylinus, the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such subunits. It is made up of two main domains, a catalytic domain (AcsA) and a regulatory domain (AcsB). AcsA and AcsB are transcribed and translated in the form of a single polypeptide Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1831
    Illegal BglII site found at 2545
    Illegal BglII site found at 4468
    Illegal BamHI site found at 841
    Illegal BamHI site found at 3835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1817
    Illegal AgeI site found at 1894
    Illegal AgeI site found at 2450
    Illegal AgeI site found at 3251
    Illegal AgeI site found at 3343
    Illegal AgeI site found at 3449
  • 1000
    COMPATIBLE WITH RFC[1000]