Difference between revisions of "Part:BBa K1493702"

Revision as of 10:26, 20 October 2014

Toggle switch part with reporter gene gfp

Part of the BananaGuard toggle switch containing a gfp reporter gene. Combined with the BioBrick BBa_K1493703 this part forms a toggle switch. This BioBrick is tested as the whole toggle switch. The part is assembled with BBa_K1493703 in a pSB3K3 backbone.

http://2014.igem.org/File:Characterization_Toggle_Switch.png

‘’Figure 1. The relative fluorescence unit of each toggle switch state. Fluorescence is measured in duplo of cell cultures carrying the pSB3K3 plasmid with the toggle switch construct (BBa_K1493702, BBa_K1493703) grown in M9 medium containing 500 ng/ml aTc (green), 2 mM IPTG (red) and with no inducer added to the medium (blue).’’

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1227
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1903

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1493702 short</partinfo>
-Part of the Banana Guard toggle switch containing a reporter gene. For the orther part of the toggle switch that combines with this part see BBa_K1493703.
+Part of the BananaGuard toggle switch containing a gfp reporter gene. Combined with the BioBrick BBa_K1493703 this part forms a toggle switch.
+This BioBrick is tested as the whole toggle switch. The part is assembled with BBa_K1493703 in a pSB3K3 backbone.
+http://2014.igem.org/File:Characterization_Toggle_Switch.png
+‘’Figure 1. The relative fluorescence unit of each toggle switch state. Fluorescence is measured in duplo of cell cultures carrying the pSB3K3 plasmid with the toggle switch construct (BBa_K1493702, BBa_K1493703) grown in M9 medium containing 500 ng/ml aTc (green), 2 mM IPTG (red) and with no inducer added to the medium (blue).’’
 <!-- Add more about the biology of this part here