Difference between revisions of "Part:BBa K1462501"
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The GBD domain, one of our scaffold subunits, is a part of GTPase binding domain in Rattus rattus. Now we do the codon opmitization according to our host cell S.cerevisae. The GBD domain allows the assembly of our targeted protein PRK. And the domain is the first subunit of our scaffold protein. | The GBD domain, one of our scaffold subunits, is a part of GTPase binding domain in Rattus rattus. Now we do the codon opmitization according to our host cell S.cerevisae. The GBD domain allows the assembly of our targeted protein PRK. And the domain is the first subunit of our scaffold protein. | ||
| − | [[File:Gbd.png|200px|left|frame|Figure 1. Simulative structure of the GBD domain which we add the linker behind it. ]] | + | [[File:Gbd-real.png|200px|left|frame|Figure 1. Simulative structure of the GBD domain which we add the linker behind it. ]] |
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Latest revision as of 15:14, 19 October 2014
GBD domian + Linker
The GBD domain, one of our scaffold subunits, is a part of GTPase binding domain in Rattus rattus. Now we do the codon opmitization according to our host cell S.cerevisae. The GBD domain allows the assembly of our targeted protein PRK. And the domain is the first subunit of our scaffold protein.
Figure 1. Simulative structure of the GBD domain which we add the linker behind it.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 60
Illegal BamHI site found at 93
Illegal BamHI site found at 265
Illegal XhoI site found at 246 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]