Difference between revisions of "Part:BBa K1462502"

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The PDZ domain, one of our scaffold subunits, is a part of the Crk protein in Mus musculus.. Now we do the codon opmitization according to our host cell S.cerevisae. The PDZ domain allows the assembly of our targeted protein CA. And the domain is the third subunit of our scaffold protein.
 
The PDZ domain, one of our scaffold subunits, is a part of the Crk protein in Mus musculus.. Now we do the codon opmitization according to our host cell S.cerevisae. The PDZ domain allows the assembly of our targeted protein CA. And the domain is the third subunit of our scaffold protein.
  
[[File:PDZ.png|200px|left|frame|Simulative structure of the SH3 domain which we add the linker behind it.]]
+
[[File:GBD.png|200px|left|frame|Simulative structure of the SH3 domain which we add the linker behind it.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:12, 19 October 2014

PDZ domain + Linker

The PDZ domain, one of our scaffold subunits, is a part of the Crk protein in Mus musculus.. Now we do the codon opmitization according to our host cell S.cerevisae. The PDZ domain allows the assembly of our targeted protein CA. And the domain is the third subunit of our scaffold protein.

File:GBD.png
Simulative structure of the SH3 domain which we add the linker behind it.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 310
    Illegal XhoI site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]