Difference between revisions of "Part:BBa K1462502"
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The PDZ domain + linker is the fusion unit of the scaffold protein and help the targeted emzyme to bind on. So we added the linker behind it and help do the next job.
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The PDZ domain, one of our scaffold subunits, is a part of the Crk protein in Mus musculus.. Now we do the codon opmitization according to our host cell S.cerevisae. The SH3 domain allows the assembly of our targeted protein RuBisCo. And the domain is the second subunit of our scaffold protein.
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[[File:SH3.png|200px|left|frame|Simulative structure of the SH3 domain which we add the linker behind it.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:05, 19 October 2014

PDZ domain + Linker

The PDZ domain, one of our scaffold subunits, is a part of the Crk protein in Mus musculus.. Now we do the codon opmitization according to our host cell S.cerevisae. The SH3 domain allows the assembly of our targeted protein RuBisCo. And the domain is the second subunit of our scaffold protein.

[[File:SH3.png|200px|left|frame|Simulative structure of the SH3 domain which we add the linker behind it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 310
    Illegal XhoI site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]