Difference between revisions of "Part:BBa K1333201"
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[[Image:Testing how to insert image of B2H system.png|thumb|center|800px|This is a graph of B2H system]]
 
[[Image:Testing how to insert image of B2H system.png|thumb|center|800px|This is a graph of B2H system]]
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===References===
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[1] Patricia Hidalgo et al, Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain, GENES & DEVELOPMENT, 2001, 15:1007–1020.
 
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Revision as of 14:33, 19 October 2014

lambda cI-LGF2(D78A)

Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Functional Parameters

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This is a graph of B2H system

References

[1] Patricia Hidalgo et al, Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain, GENES & DEVELOPMENT, 2001, 15:1007–1020.