Difference between revisions of "Part:BBa K1529310"
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<partinfo>BBa_K1529310 short</partinfo>
 
<partinfo>BBa_K1529310 short</partinfo>
  
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We newly developed the <i>rhl(LR)</i> promoter (<partinfo>BBa_K1529310</partinfo>) which is activated by C4HSL (Fig. 1). <br>
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We newly developed the Prhl(LR) promoter (<partinfo>BBa_K1529310</partinfo>) by improving Prhl promoter (<partinfo>BBa_R0071</partinfo>) which is activated by C4HSL-RHLR complex  (Fig. 1). <br>
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To characterize the function of this <i>rhl(LR)</i> promoter, we constructed a part Prhl(LR)_GFP (<partinfo>BBa_K1529311</partinfo>) by inserting the <i>rhl(LR)</i> promoter, upstream of a GFP coding sequence.
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To characterize the function of this Prhl(LR) promoter, we constructed a part, Prhl(LR)-GFP (<partinfo>BBa_K1529311</partinfo>), by inserting the Prhl(LR) promoter, upstream of a GFP coding sequence.
  
 
[[Image:titech2014_parts_rhl_promoter_main_Fig1.png|thumb|center|550px|<b>Fig. 1.</b> Our newly designed  promoter]]
 
[[Image:titech2014_parts_rhl_promoter_main_Fig1.png|thumb|center|550px|<b>Fig. 1.</b> Our newly designed  promoter]]
 
[[Image:titech2014_parts_luxR_and_rhlR_difference_main_Fig2.png|thumb|center|550px|<b>Fig. 2.</b> Difference between LuxR and RhlR]]
 
[[Image:titech2014_parts_luxR_and_rhlR_difference_main_Fig2.png|thumb|center|550px|<b>Fig. 2.</b> Difference between LuxR and RhlR]]
  
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By using the reporter cells that contain Prhl(LR)_GFP, we measured the fluorescence intensity of the cells induced by C4HSL (Fig. 2).
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By using the reporter cells that contain Prhl(LR)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.) <br>
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We saw that our new <i>rhl(LR)</i> promoter was actually activated by C4HSL through an induction assay (Fig. 3).
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We saw that our new Prhl(LR) promoter was actually activated by C4HSL through an induction assay
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[[Image:titech2014_parts_GFP_assay_main_Fig.3.jpg|thumb|center|550px|<b>Fig. 3.</b> Fluorescence intensity detected by flow cytometer]]
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[[Image:Improved_Prhl(RL)_Promoter_Assay_Result.png|thumb|center|600px|<b>Fig. 3.</b> Fluorescence intensity detected by flow cytometer]]
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1529310 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1529300 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1529310 parameters</partinfo>
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<partinfo>BBa_K1529300 parameters</partinfo>
 
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Revision as of 11:44, 19 October 2014

Prhl(LR)

We newly developed the Prhl(LR) promoter (BBa_K1529310) by improving Prhl promoter (BBa_R0071) which is activated by C4HSL-RHLR complex (Fig. 1).
To characterize the function of this Prhl(LR) promoter, we constructed a part, Prhl(LR)-GFP (BBa_K1529311), by inserting the Prhl(LR) promoter, upstream of a GFP coding sequence.

Fig. 1. Our newly designed promoter
Fig. 2. Difference between LuxR and RhlR

By using the reporter cells that contain Prhl(LR)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.)
We saw that our new Prhl(LR) promoter was actually activated by C4HSL through an induction assay

Fig. 3. Fluorescence intensity detected by flow cytometer


For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]