Difference between revisions of "Part:BBa K1403015"
Line 4: Line 4:
 
<ul>
 
<ul>
 
<li>Constitutive promoter <partinfo>BBa_J23108</partinfo></li>
 
<li>Constitutive promoter <partinfo>BBa_J23108</partinfo></li>
−
<li>sRBS</partinfo></li>
+
<li>Synthetic RBS</li>
−
<li>Histidine tag</partinfo></li>
+
<li>Histidine tag</li>
  
  
 
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===Usage and Biology===
 
===Usage and Biology===
 +
Bacterial trimethylamine moonoxygenase is similar to mammalian flavin-containing monooxygenase 3 (FMO3) which oxidizes trimethylamine (fish odor) into non-smelly trimethylamine oxide. It also produces indigo (blue pigment) form tryptophan.
  
 
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Revision as of 05:08, 19 October 2014

Trimethylamine moonoxygenase (tmm) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • Histidine tag


    • Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 7
      Illegal NheI site found at 30
    • 21
      INCOMPATIBLE WITH RFC[21]
      Illegal BamHI site found at 438
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal AgeI site found at 262
      Illegal AgeI site found at 485
    • 1000
      COMPATIBLE WITH RFC[1000]