Difference between revisions of "Part:BBa K1331002"

Revision as of 04:57, 19 October 2014

RhlR regulatory protein from P. aeruginosa SQ6

This part encodes a transcription regulatory protein RhlR.

It includes the whole coding sequence and a part of downstream noncoding sequence of rhlR.

The purpose of remaining the noncoding sequence is for composition with our part BBa_K1331003. See our composite part rhlRI (BBa_K1331007).

Usage and Biology

RhlR is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase I.

It also functions as a transcriptional activator of elastase structural gene (lasB).

RhlR binds to autoinducer molecules C4-HSL which is synthesized by rhlI, and the complex positively regulates the promoter of rhlAB consequently, while rhlR alone negtively regulates the rhlAB promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 863
Illegal BamHI site found at 344
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 843
Illegal NgoMIV site found at 847
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part encodes a transcription regulatory protein RhlR.
-It includes the whole coding sequence and a part of downstream noncoding sequence of RhlR.
+It includes the whole coding sequence and a part of downstream noncoding sequence of rhlR.
 The purpose of remaining the noncoding sequence is for composition with our part [https://parts.igem.org/Part:BBa_K1331003 BBa_K1331003]. See our composite part rhlRI ([https://parts.igem.org/Part:BBa_K1331007 BBa_K1331007]).