Difference between revisions of "Part:BBa K1403019"
Line 4: Line 4:
 
<li>Constitutive T7 promoter
 
<li>Constitutive T7 promoter
 
<li>RBS
 
<li>RBS
−
<li>CDS: Acetohydroxybutanoate synthase/acetolactate synthase (IlvB/N) from <i>E. coli</i> MG1655
+
<li>CDS: <i>IlvB/N</i> from <i>E. coli</i> MG1655
 
</ul>
 
</ul>
  

Revision as of 04:16, 19 October 2014

Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette

  • Constitutive T7 promoter
  • RBS
  • CDS: IlvB/N from E. coli MG1655

Transform E. coli to overexpress ilvB and thus increase the basal production of alpha acetolactate, the precursor of acetoin.

  • This was submitted in a pETDuet-1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]