Difference between revisions of "Part:BBa K1403017"
Line 5: Line 5:
 
<li>Constitutive promoter <partinfo>BBa_J23108</partinfo></li>
 
<li>Constitutive promoter <partinfo>BBa_J23108</partinfo></li>
 
<li>Synthetic RBS</li>
 
<li>Synthetic RBS</li>
−
<li>CDS: AldB from <i> Lactococcus lactis</i></li>
+
<li>CDS: <i>aldB</i> from <i> Lactococcus lactis</i></li>
 
<li>Terminators <partinfo>BBa_B0010</partinfo> & <partinfo>BBa_B0012</partinfo></li>
 
<li>Terminators <partinfo>BBa_B0010</partinfo> & <partinfo>BBa_B0012</partinfo></li>
 
</ul>
 
</ul>

Revision as of 04:15, 19 October 2014

Acetaldehyde dehydrogenase (aldB) expression cassette

Transform E. coli to produce acetoin.
AldB requires alpha acetolactate as substrate. A co-transformation with part BBa_K1403019 increases the amount of intracellular alpha acetolactate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Verification

AldB.png

Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter