Difference between revisions of "Part:BBa K1331007"
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It includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI. | It includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI. | ||
| − | The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system. | + | The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system of rhlAB transcription. |
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Revision as of 04:13, 19 October 2014
Modified rhlRI coding sequence from Pseudomonas aeruginosa SQ6
This part is combined with BBa_K1331002 and BBa_K1331003.
It includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI.
The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system of rhlAB transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 863
Illegal BamHI site found at 344 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 843
Illegal NgoMIV site found at 847
Illegal NgoMIV site found at 994 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1126