Difference between revisions of "Part:BBa K1403009"
Line 3: Line 3:
  
 
<ul>
 
<ul>
−
<li>Constitutive promoter <i>BBa_J23108</i></li>
+
<li>Constitutive promoter <partinfo>BBa_J23108</partinfo></li>
 
<li>Synthetic RBS</li>
 
<li>Synthetic RBS</li>
 
<li>CDS: BSMT1 from <i>Petunia × hybrida</i></li>
 
<li>CDS: BSMT1 from <i>Petunia × hybrida</i></li>

Revision as of 04:11, 19 October 2014

Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: BSMT1 from Petunia × hybrida
  • Histidine Tag

Transform E. coli and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]