Difference between revisions of "Part:BBa K1403003"
Line 3: Line 3:
  
 
<ul>
 
<ul>
−
<li>Constitutive promoter BBa_J23108</li>
+
<li>Constitutive promoter <partinfo>BBa_J23108</partinfo></li>
 
<li>Synthetic RBS</li>  
 
<li>Synthetic RBS</li>  
 
<li>CDS: LIMS1 from <i>Citrus limon</i></li>  
 
<li>CDS: LIMS1 from <i>Citrus limon</i></li>  

Revision as of 04:07, 19 October 2014

Limonene synthase (LIMS1) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: LIMS1 from Citrus limon

Transform E. coli for (+)-Limonene production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Verification

Limonene.png

Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator