Difference between revisions of "Part:BBa K1493701:Design"

 
 
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<partinfo>BBa_K1493701 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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This Biobrick is designed as read-trough control for the [http://2014.igem.org/Team:Wageningen_UR/project/characterization rhamnose mediated characterization].
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The system was designed as a tow plasmid system, but there was no other low coppy plasmid available than pSB1K3 ([http://2014.igem.org/Team:Wageningen_UR/project/low_copy low copy plasmids]). This plasmid was then designed to correct for read trough GFP expression.
  
  

Latest revision as of 22:21, 18 October 2014

pCI/Lac + TetR + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1589


Design Notes

This Biobrick is designed as read-trough control for the [http://2014.igem.org/Team:Wageningen_UR/project/characterization rhamnose mediated characterization].

The system was designed as a tow plasmid system, but there was no other low coppy plasmid available than pSB1K3 ([http://2014.igem.org/Team:Wageningen_UR/project/low_copy low copy plasmids]). This plasmid was then designed to correct for read trough GFP expression.


Source

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References