Difference between revisions of "Part:BBa K1526009"
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<p>This Biobrick has been created in order to facilitate the usage of lac repressor operon on ''E. coli'' strains that does not posses it (i.e. "E. coli DH5A"). Until this moment there was no functional complete lac repressor in the registry.</p> | <p>This Biobrick has been created in order to facilitate the usage of lac repressor operon on ''E. coli'' strains that does not posses it (i.e. "E. coli DH5A"). Until this moment there was no functional complete lac repressor in the registry.</p> | ||
<p> | <p> | ||
| − | + | We have cloned the Lac repressor system upstream a GFP Biobrick in order to observe the functionality of our part under induction of IPTG.</p> | |
<p> | <p> | ||
| − | + | Our positive results have helped to improve the characterization of this Biobrick's main component: [https://parts.igem.org/Part:BBa_P0312 BBa_P0312]. Up to this date there was no experimental data for BBa_P0312 proving its functionality.</p> | |
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Latest revision as of 18:08, 18 October 2014
Lac repressor System (LacRS)
Lac Respressor System
This Biobrick has been created in order to facilitate the usage of lac repressor operon on E. coli strains that does not posses it (i.e. "E. coli DH5A"). Until this moment there was no functional complete lac repressor in the registry.
We have cloned the Lac repressor system upstream a GFP Biobrick in order to observe the functionality of our part under induction of IPTG.
Our positive results have helped to improve the characterization of this Biobrick's main component: BBa_P0312. Up to this date there was no experimental data for BBa_P0312 proving its functionality.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1207
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]