Difference between revisions of "Part:BBa K1526008"

 
 
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<partinfo>BBa_K1526008 short</partinfo>
 
<partinfo>BBa_K1526008 short</partinfo>
  
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LacI + CysP
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Lac Repressor System + CysP
  
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The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family
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===Usage and Biology===
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<b>Gene:</b> cysP (AKA YlnA) <br>
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<b>Organism:</b> <i>Bacillus Subtilis</i> <br>
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<b>Protein:</b> CysP <br>
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<b>Function:</b> This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space. <br>
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<b>Aim:</b> We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium. <br>
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<b>Expression and characterization:</b> In order to characterise the sulfate secondary transporter from <i>Bacillus subtilis</i> we have acquired <i>E. coli BW25113</i> strain mutants from the Keio collection for complementary assays.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 18:05, 18 October 2014

LacRS + CysP

Lac Repressor System + CysP

The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family

Gene: cysP (AKA YlnA)
Organism: Bacillus Subtilis
Protein: CysP
Function: This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space.
Aim: We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium.
Expression and characterization: In order to characterise the sulfate secondary transporter from Bacillus subtilis we have acquired E. coli BW25113 strain mutants from the Keio collection for complementary assays. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2102
    Illegal NgoMIV site found at 2220
  • 1000
    COMPATIBLE WITH RFC[1000]