Difference between revisions of "Part:BBa K1403003"
Line 5: | Line 5: | ||
<li>Constitutive promoter BBa_J23108</li> | <li>Constitutive promoter BBa_J23108</li> | ||
<li>Synthetic RBS</li> | <li>Synthetic RBS</li> | ||
− | <li>LIMS1 from <i>Citrus limon</i></li> | + | <li>CDS: LIMS1 from <i>Citrus limon</i></li> |
</ul> | </ul> | ||
Revision as of 16:36, 18 October 2014
Limonene synthase (LIMS1) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: LIMS1 from Citrus limon
Transform E. coli for (+)-Limonene production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 187
Verification
Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator