Difference between revisions of "Part:BBa K1526009:Experience"
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<p>In order to characterize the parts we have created a new BioBrick compatible with our chassis. As | <p>In order to characterize the parts we have created a new BioBrick compatible with our chassis. As | ||
<i>E.coli DH5α</i> does not have a lac repressor system we have assembled together basic parts J32100, B0033, C0012, B0010, B0012 and R0010 to create the composite regulator Lac Repressor System (LacRS)</p> | <i>E.coli DH5α</i> does not have a lac repressor system we have assembled together basic parts J32100, B0033, C0012, B0010, B0012 and R0010 to create the composite regulator Lac Repressor System (LacRS)</p> | ||
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| − | <p>We have successfully cloned and tested the functionality of LacRS in pSB1A3 (<a href="https://parts.igem.org/Part:BBa_K1526009">BBa_K1526009</a>) into <i>E.coli DH5α</i> | + | <p>We have successfully assembled the parts together by step-wise 3A Assemblies, transformations and gel checking for expected sizes. |
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| + | <p><p>We have successfully cloned and tested the functionality of LacRS in pSB1A3 (<a href="https://parts.igem.org/Part:BBa_K1526009">BBa_K1526009</a>) into <i>E.coli DH5α</i> | ||
</p> | </p> | ||
| − | [[File:Plates_LacRS_I.jpg]] | + | <p>Our first functionalization test coupling LacRS upstream the Green Fluorescent Protein (GFP) Biobrick (E0040) showed expression of GFP under UV light when the system is previously induced by IPTG.</p> |
| + | [[File:Plates_LacRS_I.jpg]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:22, 18 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Creating the regulator LacRS
In order to characterize the parts we have created a new BioBrick compatible with our chassis. As E.coli DH5α does not have a lac repressor system we have assembled together basic parts J32100, B0033, C0012, B0010, B0012 and R0010 to create the composite regulator Lac Repressor System (LacRS)
We have successfully assembled the parts together by step-wise 3A Assemblies, transformations and gel checking for expected sizes. <p><p>We have successfully cloned and tested the functionality of LacRS in pSB1A3 (<a href="https://parts.igem.org/Part:BBa_K1526009">BBa_K1526009</a>) into E.coli DH5α
Our first functionalization test coupling LacRS upstream the Green Fluorescent Protein (GFP) Biobrick (E0040) showed expression of GFP under UV light when the system is previously induced by IPTG.
User Reviews
UNIQa486beadde7b79f6-partinfo-00000000-QINU UNIQa486beadde7b79f6-partinfo-00000001-QINU