Difference between revisions of "Part:BBa K1526009:Experience"
(→Applications of BBa_K1526009) |
(→User Reviews) |
||
| Line 18: | Line 18: | ||
<!-- DON'T DELETE --><partinfo>BBa_K1526009 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1526009 StartReviews</partinfo> | ||
<!-- Template for a user review | <!-- Template for a user review | ||
| − | {|width=' | + | {|width='30%' style='border:1px solid gray' |
|- | |- | ||
|width='10%'| | |width='10%'| | ||
Revision as of 16:13, 18 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Creating the regulator LacRS
In order to characterize the parts we have created a new BioBrick compatible with our chassis. As E.coli DH5α does not have a lac repressor system we have assembled together basic parts J32100, B0033, C0012, B0010, B0012 and R0010 to create the composite regulator Lac Repressor System (LacRS)
<img src="
" style="border:1px solid orange">
Our first functionalization test coupling LacRS upstream the Green Fluorescent Protein (GFP) Biobrick (E0040) showed expression of GFP under UV light when the system is previously induced by IPTG.
We have successfully cloned and tested the functionality of LacRS in pSB1A3 (<a href="https://parts.igem.org/Part:BBa_K1526009">BBa_K1526009</a>) into E.coli DH5α
<img src=
class="img-responsive" style="width:100%; border:3px solid orange;">
User Reviews
UNIQ50415617bd15c02c-partinfo-00000000-QINU UNIQ50415617bd15c02c-partinfo-00000001-QINU