Difference between revisions of "Part:BBa K1403019"
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| − | Transform <i>E. coli</i> to overexpress <i>ilvB< | + | Transform <i>E. coli</i> to overexpress <i>ilvB</i> and thus increase the basal production of alpha acetolactate, the precursor of acetoin. |
* This was submitted in a pETDuet-1 | * This was submitted in a pETDuet-1 | ||
Revision as of 16:05, 18 October 2014
Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette
- Constitutive T7 promoter
- RBS
- CDS: Acetohydroxybutanoate synthase/acetolactate synthase (IlvB/N) from E. coli MG1655
Transform E. coli to overexpress ilvB and thus increase the basal production of alpha acetolactate, the precursor of acetoin.
- This was submitted in a pETDuet-1
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
- 1000COMPATIBLE WITH RFC[1000]