Difference between revisions of "Part:BBa K1403017"
Line 3: | Line 3: | ||
<ul> | <ul> | ||
− | <li>Constitutive promoter BBa_J23108 | + | <li>Constitutive promoter BBa_J23108</li> |
− | <li>Synthetic RBS | + | <li>Synthetic RBS</li> |
− | <li>CDS: | + | <li>CDS: alpha acetolactate decarboxylase (aldB) from <i> Lactococcus lactis</i></li> |
− | <li>Terminators BBa_B0010 & BBa_B0012 | + | <li>Terminators BBa_B0010 & BBa_B0012</li> |
</ul> | </ul> | ||
Transform <i>E. coli</i> to produce acetoin. | Transform <i>E. coli</i> to produce acetoin. | ||
− | The enzyme requires | + | The enzyme requires alpha acetolactate. |
A co-transformation with part BBa_K1403019 increases the amount of substrate for aldB. | A co-transformation with part BBa_K1403019 increases the amount of substrate for aldB. | ||
Revision as of 16:04, 18 October 2014
Acetaldehyde dehydrogenase (aldB) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: alpha acetolactate decarboxylase (aldB) from Lactococcus lactis
- Terminators BBa_B0010 & BBa_B0012
Transform E. coli to produce acetoin. The enzyme requires alpha acetolactate. A co-transformation with part BBa_K1403019 increases the amount of substrate for aldB.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]
Verification
Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter