Difference between revisions of "Part:BBa K1526009"

 
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<partinfo>BBa_K1526009 short</partinfo>
 
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Lac operon
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Lac Respressor System
  
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This Biobrick has been created in order to facilitate the usage of lac repressor operon on ''E. coli'' strains that does not posses it (i.e. "E. coli DH5A"). Until this moment there was no functional complete lac repressor in the registry.
===Usage and Biology===
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We have put the parts from the Lac repressor system upstream a GFP Biobrick in order to observe the functionality of our part under induction of IPTG.
  
 
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Revision as of 16:04, 18 October 2014

Lac repressor System (LacRS)

Lac Respressor System

This Biobrick has been created in order to facilitate the usage of lac repressor operon on E. coli strains that does not posses it (i.e. "E. coli DH5A"). Until this moment there was no functional complete lac repressor in the registry. We have put the parts from the Lac repressor system upstream a GFP Biobrick in order to observe the functionality of our part under induction of IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]