Difference between revisions of "Part:BBa K1526006"
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<b>This Biobrick was created in order to allow ''SpPCS'' gene characterization in '''''E. coli DH5A'''''<br> | <b>This Biobrick was created in order to allow ''SpPCS'' gene characterization in '''''E. coli DH5A'''''<br> | ||
− | + | Gene:</b> ''spPCS''<br> | |
<b>Organism:</b> <i>Schizosaccharomyces pombe</i><br> | <b>Organism:</b> <i>Schizosaccharomyces pombe</i><br> | ||
<b>Function:</b> The phytochelatin synthase in ''S. pombe'' uses glutathione with a blocked thiol group to synthesise phytochelatins.<br> | <b>Function:</b> The phytochelatin synthase in ''S. pombe'' uses glutathione with a blocked thiol group to synthesise phytochelatins.<br> |
Latest revision as of 15:54, 18 October 2014
LacRS + SpPCS
Lac Repressor System + SpPCS
This Biobrick was created in order to allow SpPCS gene characterization in E. coli DH5A
Gene: spPCS
Organism: Schizosaccharomyces pombe
Function: The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group to synthesise phytochelatins.
Aim: Over-expression of this gene, together with Gsh1*, in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in taking up cadmium.
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1207
Illegal BamHI site found at 1879 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]