Difference between revisions of "Part:BBa K1526006"

Revision as of 15:53, 18 October 2014

LacRS + SpPCS

Lac Repressor System + SpPCS

This Biobrick was created in order to allow SpPCS gene characterization in E. coli DH5A <b>Gene: spPCS
Organism: Schizosaccharomyces pombe
Function: The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group to synthesise phytochelatins.
Aim: Over-expression of this gene, together with Gsh1*, in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in taking up cadmium.

Literature

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1207
Illegal BamHI site found at 1879
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1526006 short</partinfo>
-lacI + SpPCS
+Lac Repressor System + ''SpPCS''
-<!-- Add more about the biology of this part here
+<b>This Biobrick was created in order to allow ''SpPCS'' gene characterization in ''E. coli DH5A''
-===Usage and Biology===
+<b>Gene:</b> ''spPCS''<br>
+<b>Organism:</b> <i>Schizosaccharomyces pombe</i><br>
+<b>Function:</b> The phytochelatin synthase in ''S. pombe'' uses glutathione with a blocked thiol group  to synthesise phytochelatins.<br>
+<b>Aim:</b> Over-expression of this gene, together with Gsh1*, in <i>E.coli</i> has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in  taking up cadmium.<br>
+<b>Literature</b><ol><li>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/</li></ol>
 <!-- -->