Difference between revisions of "Part:BBa K1526003"

Latest revision as of 15:45, 18 October 2014

CysP from Bacillus subtilis

The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family

Gene: cysP (AKA YlnA)
Organism: Bacillus Subtilis
Protein: CysP
Function: This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space.
Aim: We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium.
Expression and characterization: In order to characterise the sulfate secondary transporter from Bacillus subtilis we have acquired E. coli BW25113 strain mutants from the Keio collection for complementation assays.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 680
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 <b>Function:</b> This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space. <br>
 <b>Aim:</b> We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium. <br>
-<b>Expression:</b> We could run assays measuring the concentration of sulfate in the environment containing our bacteria.
+<b>Expression and characterization:</b> In order to characterise the sulfate secondary transporter from <i>Bacillus subtilis</i> we have acquired <i>E. coli BW25113</i> strain mutants from the Keio collection for complementation assays.
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