Difference between revisions of "Part:BBa K1526003"

Revision as of 15:43, 18 October 2014

CysP from Bacillus subtilis

The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family

Gene: cysP (AKA YlnA)
Organism: Bacillus Subtilis
Protein: CysP
Function: This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space.
Aim: We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium.
Expression: We could run assays measuring the concentration of sulfate in the environment containing our bacteria.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 680
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1526003 short</partinfo>
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 The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family
-<!-- Add more about the biology of this part here
+<b>Gene:</b> cysP (AKA YlnA) <br>
-===Usage and Biology===
+<b>Organism:</b> <i>Bacillus Subtilis</i> <br>
+<b>Protein:</b> CysP <br>
+<b>Function:</b> This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space. <br>
+<b>Aim:</b> We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium. <br>
+<b>Expression:</b> We could run assays measuring the concentration of sulfate in the environment containing our bacteria.
 <!-- -->