Difference between revisions of "Part:BBa K1526000"
 
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<partinfo>BBa_K1526000 short</partinfo>
 
<partinfo>BBa_K1526000 short</partinfo>
  
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The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group in the synthesis of phytochelatin. Overexpression of this gene, together with Gsh1* (BBa_K1526001), in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/).  
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The phytochelatin synthase gene from ''Schizosaccharomyces  pombe''.
  
  
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<b>Function:</b> The phytochelatin synthase in ''S. pombe'' uses glutathione with a blocked thiol group  to synthesise phytochelatins.<br>
 
<b>Function:</b> The phytochelatin synthase in ''S. pombe'' uses glutathione with a blocked thiol group  to synthesise phytochelatins.<br>
 
<b>Aim:</b> Over-expression of this gene, together with Gsh1*, in <i>E.coli</i> has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in  taking up cadmium.<br>
 
<b>Aim:</b> Over-expression of this gene, together with Gsh1*, in <i>E.coli</i> has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in  taking up cadmium.<br>
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<b>Literature</b><ol><li>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/</li></ol></p></div>
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<b>Literature</b><ol><li>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/</li></ol>
  
 
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Latest revision as of 15:41, 18 October 2014

SpPCS: Phytochelatin synthase from Schizosaccharomyces pombe

The phytochelatin synthase gene from Schizosaccharomyces pombe.


Gene: spPCS
Organism: Schizosaccharomyces pombe
Function: The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group to synthesise phytochelatins.
Aim: Over-expression of this gene, together with Gsh1*, in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in taking up cadmium.

Literature
  1. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]