Difference between revisions of "Part:BBa K1403017"
Line 2: | Line 2: | ||
<partinfo>BBa_K1403017 short</partinfo> | <partinfo>BBa_K1403017 short</partinfo> | ||
− | aldB | + | <ul> |
+ | <li>Constitutive promoter BBa_J23108 | ||
+ | <li>Synthetic RBS | ||
+ | <li>CDS: &945;-acetolactate decarboxylase (aldB) | ||
+ | <li>Terminators BBa_B0010 & BBa_B0012 | ||
+ | </ul> | ||
− | + | Transform <i>E. coli</i> to produce acetoin. | |
+ | The enzyme requires &945;-acetolactate. | ||
+ | A co-transformation with part BBa_K1403019 increases the amount of substrate for aldB. | ||
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Revision as of 15:36, 18 October 2014
Acetaldehyde dehydrogenase (aldB) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: &945;-acetolactate decarboxylase (aldB)
- Terminators BBa_B0010 & BBa_B0012
Transform E. coli to produce acetoin. The enzyme requires &945;-acetolactate. A co-transformation with part BBa_K1403019 increases the amount of substrate for aldB.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]
Verification
Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter