Difference between revisions of "Part:BBa K1526007"
 
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<b>Aim:</b> We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.<br>
 
<b>Aim:</b> We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.<br>
 
<b>Expression:</b> We can run assays measuring the concentration of cadmium in the environment. If ''MntH'' is active, the concentration of cadmium should decrease.
 
<b>Expression:</b> We can run assays measuring the concentration of cadmium in the environment. If ''MntH'' is active, the concentration of cadmium should decrease.
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<b>Mutant strains:</b> Information about mutant ''E. coli'' strains for ''MntH'' can be found at <a href="http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909">EchoBase</a> website from the University of York.
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<b>Mutant strains:</b> Information about mutant ''E. coli'' strains for ''MntH'' can be found at EchoBase website from the University of York (http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909).
  
  

Latest revision as of 15:35, 18 October 2014

LacRS + MntH

MntH gene under control of Lac Repressor System

Gene: mntH
Organism: Escherichia Coli
Protein: MntH transporter from NRAMP family
Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.
Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.
Expression: We can run assays measuring the concentration of cadmium in the environment. If MntH is active, the concentration of cadmium should decrease. Mutant strains: Information about mutant E. coli strains for MntH can be found at EchoBase website from the University of York (http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
    Illegal BamHI site found at 2462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2358
  • 1000
    COMPATIBLE WITH RFC[1000]