Difference between revisions of "Part:BBa K1526007"

Revision as of 15:29, 18 October 2014

LacRS + MntH

MntH gene under control of Lac Repressor System

Gene: mntH
Organism: Escherichia Coli
Protein: MntH transporter from NRAMP family
Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.
Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.
Expression: We can run assays measuring the concentration of cadmium in the environment. If MntH is active, the concentration of cadmium should decrease. Mutant strains: Information about mutant E. coli strains for MntH can be found at <a href="http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909">EchoBase</a> website from the University of York.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1188
Illegal BamHI site found at 2462
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2358
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1526007 short</partinfo>
-lacI + NRAMP
+''MntH'' gene under control of Lac Repressor System
+<b>Gene:</b> mntH<br>
+<b>Organism:</b> <i>Escherichia Coli</i><br>
+<b>Protein:</b> ''MntH'' transporter from NRAMP family<br>
+<b>Function:</b> NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.<br>
+<b>Aim:</b> We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.<br>
+<b>Expression:</b> We can run assays measuring the concentration of cadmium in the environment. If ''MntH'' is active, the concentration of cadmium should decrease.
+<b>Mutant strains:</b> Information about mutant ''E. coli'' strains for ''MntH'' can be found at <a href="http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909">EchoBase</a> website from the University of York.
-<!-- Add more about the biology of this part here
-<p>Gene: mntH
-<p>Organism: Escherichia Coli
-<p>Protein: MntH transporter from NRAMP family
-<p>Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.
-<p>Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.
-Expression: We can run assays measuring the concentration of cadmium in the environment. If NRAMP is active, the concentration of cadmium should decrease.
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